产品货号：
GS2141
中文名称：
BstH2I内切酶
英文名称：
BstH2I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：BstH2I内切酶，BstH2I限制性内切酶
使用说明
High enzyme concentration may result in star activity To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | RGCGC↑Y |
| 来源 | Bacillus stearothermophilus H2 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM KH2PO4(pH 7.2);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BfoI,Bsp143II,HaeII |
相关搜索：BstH2I内切酶，BstH2I限制性内切酶